Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNILVLGSGGREHALAWKIAQDAKVTQVFVAPGNA--GTATEPKCVNVQLDILDNPAIIAFAKENNVELIVVGPEAPLVNGVVDAAREAGVKIWGPTQYAAQLEGSKAFAKHFLKRHNIPTAFYEVFTEVDAAKAYVEKNGAP-IVIKADGLAAGKGVIVAMTNEEAFAAIDDMLAGNKFGDAGSRVVIEQFLAGEEASFICMIDGKNILPMATSQDHKRIFEGDQGPNTGGMGAYSPAPVVTPDVFERVMNEIMRP-TVDGMAADGH---VYTGFLYAGLMIDEQGQPRVIEFNCRFGDPETQPIMMRLKSSLVDLVEAGIAGNLPSE-AEWDE-RKSIGIVLAAEGYPDSVRKGDVISGIGQSP-EDTKIFHAGTATREDGHIVTSGGRVLCVTALGDSVLEAQINALEVCGQVTFTGMQYRSDIGYRAIAREKAE 2QK4 Chain:A ((25-449)) ARVLIIGSGGREHTLAWKLAQSHHVKQVLVAPGNAGTACSEKISNTAISIS--DHTALAQFCKEKKIEFVVVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSADFPALVVKASGL---KGVIVAKSKEEACKAVQEIMQE-------ETIVIEELLDGEEVSCLCFTDGKTVAPMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSNDLLL----KIKDTVLQRTVDGMQQEGTPYTGILYAGIMLTK-NGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTLDGLLCTSLPVWLENHTALTVVMASKGYPGDYTKGVEITGFPEAQALGLEVFHAGTALKNG-KVVTHGGRVLAVTAIRENLISALEEAKKGLAAIKFEGAIYRKDIGFRAIA-----

General information: TITO was launched using: RESULT: Template: 2QK4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2427 9743 4.01 24.06 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : 4.01 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_2QK4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QK4-query.scw PDB file : Tito_Scwrl_2QK4.pdb: