Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKTLQFGITNFEIDFKDLVMNTMIKNKRFENDVEFRGADIKDSRAVVILLHGRRQNADTIYELAEKIGFTDITYILPLAPELTWYPRGFMRD-----LEDNQPYLSVALERIHYIITDLKNQNIKPENIFLMGFSQGACIVSQYLWENPQKLGGVIAFTGGLFGPELTYT------------SHE----GLPINGTQLIFTGGKEDSWVPEQRVRDTAALFEKLGG--NVTTKIYSGRDHLVSDEEVELAKTLLNI 1UFO Chain:B ((14-232)) --------------------------------SVLARIPE--APKALLLALHGLQGSKEHILALLPGYAERGFLLLAFDAPRHGEREGPPPSSKSPRYVEEVYRVALGFKEEARRVAEEAERR--FGLPLFLAGGSLGAFVAHLLLAEGF-RPRGVLAFIGSGFPMKLPQGQVVEDPGVLALYQAPPATRGEAYGGVPLLHLHGSRDHIVPLARMEKTLEALRPHYPEGRLARFVEEGAGHTLTPLMARVGLAFLE-

General information: TITO was launched using: RESULT: Template: 1UFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1037 -44045 -42.47 -224.72 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -42.47 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_1UFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UFO-query.scw PDB file : Tito_Scwrl_1UFO.pdb: