Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFQVSTLSQKNHVIIQITDTHLLEYPQLE--------------------------F-----VGMNPEESFHAIIQQILKQHPEADAIIHTGDLAQAPTP--------ITYKRYINFMQTL--GLPFFQTLGNHDNVDHFP----------------LHN--------EN-------HQQPVVVG-LGNWRVIMLNSAVKGK------VDGHLSSEQLENLANLLEQF--ADHPVLLACHHHPFAMKSKWIDHHKLQNSNALLDTLSPFQ-NVKALVCGHVHQDSLNIW-QG-------VEFFSTPSTSVQFKPFSNDFALDQNAPGYRYIRLNNDGSFETKVFRLENFKTRINTDISGY 5FIB Chain:A ((119-428)) -------PGAPVSRVLFLTDLHWDHEYLEGTDPYCADPLCCRRGSGWPPNSQKGAGFWGEYSKCDLPLRTLESLLKGLG-PAGPFEMVYWTGDIPAHDVWQQSRQDQLRALTTITDLVRKFLGPVPVYPAVGNHESTPVNGFPPPFIKGNQSSQWLYEAMAKAWEPWLPADALHTLRIGGFYALTPRPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVEELQAAENRGDKVHIIGHIPPGHCLKS--------WSWNYYKIIARYENTLAGQFFGHTHVDEFEIFYDEETLSRPLAVAFLAPSATTF----------INLNPGYRVYQIDGN------------------------

General information: TITO was launched using: RESULT: Template: 5FIB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1020 -9162 -8.98 -41.65 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -8.98 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_5FIB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FIB-query.scw PDB file : Tito_Scwrl_5FIB.pdb: