Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGQYRVTALIPARGGSKRLPRKNVKLLVDKPLIAWSIEVAKASKYIDRVVVSTDDEEIKQVSEQYGAEVPFLRPEYLSNDHASSFDVIKHAIDFLHLGQKNELIVLLQPTSPLRLVSELDTALEFFIAKNAKGIVSISETEHSP------MWSNTL-PENGCMSDFIRP--EVQGK---RSQD-----LPKFFRLNGSIYIYETLCLLEQ-----S-----K----I--FF-NENVYGFETSLKTAIDIDTDLDFLIAETIMKNRAIE 1VIC Chain:A ((2-254)) -----SFTVIIPARFASSRLPGKPLADIKGKPMIQHVFEKALQSG-ASRVIIATDNENVADVAKSFGAEVCMTSVNHNS-GTE----RLAEVVEKLAI-PDNEIIVNIQGDEPLIPPVIVRQVADNLAKFNVNMASLAVKIH-DAEELFNPNAVKVLTDKDGYVLYFSRSVIPYDRDQFMNLQDVQKVQLSDAYLRHIGIYAYRAGFIKQYVQWAPTQLENLEKLEQLRVLYNGERIHVELAKEVPAVGVDTAEDLEKVRAILAAN---

General information: TITO was launched using: RESULT: Template: 1VIC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1028 -53222 -51.77 -243.02 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -51.77 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.444

(partial model without unconserved sides chains): PDB file : Tito_1VIC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VIC-query.scw PDB file : Tito_Scwrl_1VIC.pdb: