Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAHLVLASSSPRRRELLQQ-LGL---NFEIYSPDIDESVHEGELVHQYVERLAREKAQAVLNI----FPDSVIIAADTSLGLDGQIIGKPDSKQHAFDIWKQLSGRWHD-VFS-GICIATQQHILSQVVQTQVEFASLTTQDMEDYWATGEPVGKAGAYAIQGIA-SQYIPKIQGSYSNVVGLPLYEFSQLFKRVKT 2AMH Chain:A ((10-207)) -RTMIIGTSSAFRANVLREHFGDRFRNFVLLPPDIDEKAYRAADPFELTESIARAKMKAVLEKARQH--PAIALTFDQVVVKGDEVREKPLSTEQCRSFIASYSGGGVRTVATYALCVVGTENVLVAHNETETFFSKFGDDIVERTLERGACMNSAGGLVVEDEDMSRHVVRIVGTSYGVRGMEPAVVEKLLSQL--

General information: TITO was launched using: RESULT: Template: 2AMH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 904 -358 -0.40 -1.98 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -0.40 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_2AMH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AMH-query.scw PDB file : Tito_Scwrl_2AMH.pdb: