Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQPFVLYNSEQRKKVEFVPRKEGHIDMYVCGMTVYDYCHIGHARVMVAFDYIIRFLRSQGWKVRYIRNITDI--DDKIIKRANENGETIQQLTMRFIDAMNEDAANLGCLAPDEAPKATEYIDQMQNMIGNLVNKGAAYPASNGDVYFEVTKFEKYGRLSGRKLDDMQAGASERVDVEVEKKHPFDFVLWKHAK---ENEPSWASPWGNGRPGWHIECSAMSTCCLGNHFDIHGGGSDLMFPHHENEIAQSEASTGEQYVNYWIHVGFINVDGEKMSKSLGNFFTIRDVME-KFHPEVIRYFIVSSHYRSPVNFSDVALKEAKTSL----TRFYHSFKAYQQVYGQ-------TTTETLDQSFVERFNNAMCDDFNTAEAMAVLFELNKELNRAVKEEQADQATVLYSTLRHLTNILGLVQHNVDDFLKSDIGQEALALSDAEIEDFIQQRVDAKKAKDFAKADSIRQSLLEQGVVLEDTRQGTVWRRAD 3SP1 Chain:B ((21-501)) SMILKLYNTRTKDFSELTNF--ENVKVYACGPTVYNYAHIGNFRTYIFGDLLIKTLRFLGYKVNYAMNITDIGH-----------GLTVYEISEFFTEAFFNDCRKLNIVYPDKVLVASKHIPIMIEVVKILEEKKITYFSN-GNVYFDTSCFKSYGEMAGI----------------KFKRNKTDFVLWFTNSKFKDQEMKWDSPWGFGYPSWHLECAAMNLEYFKDALDIHLGGVDHIGVHHINEIAIAECFLNKKWCDVFVHGEFLIMDYNKMS-----FITVKDLEDQNFSPLDFRYLCLTSHYRNQLKFSLDNLQASKIARENLINKLSYFYESLDPVDLNTLNKDLKNFGFSVEKEYYDSFVEKISFDLNVAQGLALLWEIIKSDNLSFVSKL--------RLAFIFDEIMSLNLREEILKNLQ----NHDVVIDENMKALIEERRIAKCEKNFKRADEIRDFFAKKGFVLVD---GTKVKRG-

General information: TITO was launched using: RESULT: Template: 3SP1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2071 -45343 -21.89 -107.45 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -21.89 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_3SP1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SP1-query.scw PDB file : Tito_Scwrl_3SP1.pdb: