Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNVDQRPIILTGDRPTGQLHLGHFVGSLRSRVGLQDSHHQHLLLADAQALTDNADNPDKVRRNILEVALDYLAVGIDPTKTTICVQSCLPALNELTMLYLNFVTVARLERNPTIKSEIQMRGFERDIPAGFLCYPVAQAADITAFKATVVPVGEDQIPMIEQTNEIVRRVNRQIGQDLLPECKALL-SNMARLPGF-DGKAKMSKSLGN---TIVLNASDKDIKKAVNAMYTDPNHLRI-EDPGQVEGNIVFTYLDAFDPNKEEVEELKAHYRRGGLGDGTVKKRLEGVLKELITPMRERREELAKDPDYIMDVLRQGTDKCRIITQQTLDEVKDGLGLFKF 1I6L Chain:A ((1-326)) ------MKTIFSGIQPSGVITIGNYIGALRQFVELQHEYNCYFCIVDQHAITVW-QDPHELRQNIRRLAALYLAVGIDPTQATLFIQSEVPAHAQAAWMLQCIVYIGELERMTQFKEKSAG---KEAVSAGLLTYPPLMAADILLYNTDIVPVGEDQKQHIELTRDLAERFNKRYGE-LFTIPEARIPKVGARIMSLVDPTKKMSKSDPNPKAYITLLDDAKTIEKKIKSAVTDSEGTIRYDKEAKPGISNLLNIYSTLSG--QSIEELERQYEGK--GYGVFKADLAQVVIETLRPIQERYHHWMES-EELDRVLDEGAEKANRVASEMVRKMEQAMGLGR-

General information: TITO was launched using: RESULT: Template: 1I6L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1609 -20048 -12.46 -62.65 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -12.46 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_1I6L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1I6L-query.scw PDB file : Tito_Scwrl_1I6L.pdb: