Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASLRSSAIPVSDPASGLRITEIFYSLQGEANASGLPTVFIRLTGCPLRCSYCDTTYSFE-------GGERLSLEHIIETA---EKYQTPYICVTGGEPLAQPNCLILLQRLCDA-GF-DVSLETSGALDVSRV-----DPRVSKVLDLKTPTSGE---------EHRNLISNLDHLTP---RDQIKFVICNREDYEWSKQQVEQYQLQTKVSTVWFSPAFAVEKGAVGLPRLARDMAQWILDDKLPVRFQLQLHKLLWNDESGR 2FB2 Chain:A ((21-183)) ------------------------------------------TDRCNFRCDYCMPKEVFGDDFVFLPKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLTTNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGLNVKVNVVIQKGINDDQIIPMLEY------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2FB2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 532 -9453 -17.77 -70.54 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -17.77 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_2FB2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FB2-query.scw PDB file : Tito_Scwrl_2FB2.pdb: