Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGMEEHLLTQKLQQRLRFSTHIQEAQAAVLIAITNENNPKVLLTRRSIHMNNHAGEVSFPGGKRDPSDTSNIVVALREAQEETALNPFDVQLLGDLPMQRARSGLSVKPIVGLIPPEVTLIPQPTEIDRIFFVPLQQLIEMRPTPYEVRYAHQSLYFPSLQIDNEIIWGLTAQMLIALFKYGLGYQKEWPFLLNAPTFGMPKFSHKK 1VHZ Chain:A ((50-162)) ---------------------------EAVMIVPIV--DDHLILIREYAV-GTESYELGFSKGLIDPGES-VYEAANRELKEEVGFGANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1VHZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 454 -40655 -89.55 -359.77 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -89.55 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.272

(partial model without unconserved sides chains): PDB file : Tito_1VHZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VHZ-query.scw PDB file : Tito_Scwrl_1VHZ.pdb: