Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNMTFIVAVQLKDSIVVAVDNKYLTLKDKEKDHFEEHFSSKLYAWHSGIITGTGEHYVIDKAVRLFINSADADIEKLPLCLNISRQIREMEVGQHEQIQSSKLLYSQYSENGAKL-FAIEPTEETGKYQRTEFKENDLIIWLFNPNIQSISGNLKTLYSNPRPKVSFD-RIEDWLDYYISAIAEIYEKQSCID-SWMSGSFDIFFQTEGEYFYKHIQNNPTVHLE----------- 1QRE Chain:A ((43-209)) ------------------SNIRENPVTPWNPEPSAPVIDPTAYIDPQASVIG---EVTIGANVMVSPM---ASIRSD--------EGMPIFVGDRSNVQDGVVLHALETINEEGEPIEDNIVEVDGKEYAVYIGNNVSLAH--------------------QSQVHGPAAVGD--DTFIGMQAFVF-KSKVGNNCVLEP-------------RSAAI-GVTIPDGRYIPAGMVVTS

General information: TITO was launched using: RESULT: Template: 1QRE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 943 -6200 -6.57 -40.52 target 2D structure prediction score : 0.22 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -6.57 2D Compatibility (Sec. Struct. Predict.) : 0.22 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.067

(partial model without unconserved sides chains): PDB file : Tito_1QRE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QRE-query.scw PDB file : Tito_Scwrl_1QRE.pdb: