Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDEEDIFLITYGDQFFEEQQPTLRTFKKFYQK--YLADT-FRVVHFLPFFPYT-SDDGFSVVNYQAINDRLGTWEDIEEMRSET-----RLMFDFVCNHMSAESTWFQEYLNL-------NDTYKDFFIDLPLDTDTS---SVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLEKGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKDNISYFGNG-ENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLASHDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSPTDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLLADIEK--------------------------ELQ-TDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSAD-SFISFIREAKEEKVLVIHNFSNQSAGYLLPK----GDYLDLFSGQII----HCSQERKLIFQPYEYMWLKIIK 4WF7 Chain:C ((8-555)) ---------------------------------------PEWYKSAVFYELSVRTFQDGNGDGKGDFPGLTSRLDYLKNLGVDCLWLLPWFPSPLRDDGYDVADYRGIHPDLGTLDDFKVFLREAHARGLWVIGDLVTNHTSSDHPWFQAARRGPTLPDGSPNEYHDYYVWSDEGKEYADTRIIFTDTEVSNWT-LDEQAG--KYYWHRFFASQPDLNYDNPKVVEELHGAARFWLDLGLDGFRVDAVPYLIEREGTSCENLPETHEILKGFRAMVDREYPGRLLLAEAAQWPEEVVEYFGTEAEPEFHMCFNFPVMPRLYMSLKREDTSSIREIMGRLPKIPSFGQWCIFLRNHDELTLEM----VTDDERAFMYAAYAPDA--------------RMKINVGIRRRLA---PLLDNDRRRIELLNTVLLALPGSPVLYYGDEIGMGDDLGL-----PDRNGVRTPMQWNAGTSGGFSTAQPSDCFFPPIQDPVYGFGRVNVQSQLQDPSSLLKWTARQLELRRAHPAFAH-GDLTFIETGNPAILAFTRQYDGETLLIVSNFAGNAQAGLLDLAPFVGRAPVTLSGASPLPVVTGNGQYPVVMGKYDYYWLRLNS

General information: TITO was launched using: RESULT: Template: 4WF7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2836 49304 17.39 100.21 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.71 3D Compatibility (PKB) : 17.39 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_4WF7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WF7-query.scw PDB file : Tito_Scwrl_4WF7.pdb: