Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------PGNRGSKLSPISSSISSPESLSDEEFEKFC----CVYIVNLGKPD-QREWPVLTGKEVWFEHKRKKYDVMAFDSCNLVWGRRGEPKGVTFEFLETRTANKDGTC----------------------------------------------------------------------------------------------------------------------------------------------------------------- 5L2X Chain:A ((1-348)) MNRKWEAKLKQIEERASPEEPPSIWRLFHRQAQAFNFVKSCKEDVHVFALECKVGDGQRIYLVTTYAEFWFYYKSRKNLLHCYEVIP-----ENAVCKLYFDLEFNKPANPGADGKKMVALLIEYVCKALQELYGVNCSAEDVLNLDSSTDEKFSRHLIFQLHDVAFKDNIHVGNFLRKILQPALDLLPDLSFLVVKNNMGEKHLFVDLGVYTRNRNFRLYKSSKIGKRVALEVTEDNKFFPIQSKDVSDEYQYFLSSLVSNVRFSDTLRILTCEP

General information: TITO was launched using: RESULT: Template: 5L2X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 257 3273 12.73 34.81 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 12.73 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.299

(partial model without unconserved sides chains): PDB file : Tito_5L2X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L2X-query.scw PDB file : Tito_Scwrl_5L2X.pdb: