Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------KCNISILKDGKYFT--QKQEIAG--WTVKFHDLDG----NYVGKCKNAHTYACTYVTCEDLAPGYSPGKSKDVVASGNVGSLGHDPSSSSPSSP 1UFF Chain:A ((1-93)) GSSGSSGYRALYPFEARNHDEMS-FNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSS-----------ENEKAVSPKKA-------LLPPTVSLSATSGPSSG

General information: TITO was launched using: RESULT: Template: 1UFF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 262 22242 84.89 331.97 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 84.89 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.444

(partial model without unconserved sides chains): PDB file : Tito_1UFF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UFF-query.scw PDB file : Tito_Scwrl_1UFF.pdb: