Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -STSTKVKLENRKEPA-------LCHVVIIKPAQIRHPLDTWVETFAWVKAGKVVEWHSPKYGSVKVKTDE---DD------CHLTFLVNELING--YTCQLSR----------- 1FNG Chain:B ((119-214)) RRVEPTVTVYPTKTQPLEHHNLLVCSVSDFYPGNIE------VR---WFRNGKEE-----KTGIVS--TGLVRNGDWTFQTLVMLETVPQS---GEVYTCQVEHPSLTDPVTVEW

General information: TITO was launched using: RESULT: Template: 1FNG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 182 11836 65.03 179.33 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : 65.03 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.631

(partial model without unconserved sides chains): PDB file : Tito_1FNG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FNG-query.scw PDB file : Tito_Scwrl_1FNG.pdb: