Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALSLEEFVYSLDLRTLPRVLEIQSGIYFEGSVYEMFGNECCLSTGEVIKITGLKIKKMMAEICEGAIGGCESQKPFELPMNFPGLFKVMADKTPYLSIEEITRTVNIGPSRLGHPCFYHLKD----IKLENLIIKQGEPIRFNSVEEINGETLVNCGVVRNHQSHSF-----TLPLSQEGEFYECEDEHIYTLKEIVEWKIPKNRTRTVKLTD-------FSNKWDSTNP----------FPEDFYGTLILKPVYEIQGVLKFQKDIVRILPSLDVEVKDITDSYDANWFLQLLSTDDLFEMTSKEFPVVAEVVEISQGNHLP---QSILQRE---------KTIVIHKKYQASRILASEIRSNFPKRHFLIPISYKGKFKRRPREFPTAYDLQIAKSRKETLHVVATKAFHTLHKELSPVSVGDQFLVHHSETTEVVFEGTRKVNVLTCEKVLNKTREDAQLPLYMEGGFVEVIHDKKQYQISELCTQFCWPFNVKVAVRDLSIKDDILAATPGLQLEEDITDSYLLISDFANPEECWEIPMSRLNMTVRLVNGSSLPADAGLLQVRSFVEEITEEQYYMMRRYESSLSHPPPRPPKHPSAEEMKLTLLSLAEERTINLPKSLKSHHVDRPKKLPSDESGQDSRAPVGFQNDVADVERQKSKHGPLQPQAPL 5A8J Chain:A ((2-366)) ----------------------------------------------------------------------------ATVSVSLKSSTNLAFTGKP-TQVSLIVRVSPTSFASLSGIHYVVMIDNSPSMKKENKINLALSSASRLVQDIIPGNFISIYLFSNDIETLYEGESGKQIELKSIKMGYT-TNLHKAITKVLEKFKSSEIPVKIILLSDGKPTDKRYSRDYESLQVPKNVQLITIGLGEDYNEAIMKILADKGSGVFYHINDPSQLPTTLVEQKSDKVAAYNLTLNFSEGLEVINYEMPVNIPVVDKDVNVYAVGNIPPGETDYTLKVTGNYVDSVSNKNIEIDESLVIKRAPDDEVRSNFD-RTVINEVSY-YMLLRYYGNLISEGKSEEATRVVQELLTAAERTKRVELIERTKKLVND-PKVDLSEVTKTMRTGSIEG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5A8J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1773 25351 14.30 77.52 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 14.30 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.096

(partial model without unconserved sides chains): PDB file : Tito_5A8J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5A8J-query.scw PDB file : Tito_Scwrl_5A8J.pdb: