Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSWKNWLIKINYCVYKKKRKGRMRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIYFREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFIAADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEGREFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQDEKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKEDNKEYSLKDLITKVSKESDNVAHNLLGYYISN-QSDATFKSKMSAIMGDD------------WDPKEKLISSKMAGKFMEAIYNQN-------GFVLESLTKTDFDSQRIAKGVS--VKVAHKIGDAD-EFKHDTGVVYAD-S-PFILSIFTKNSDY------DTISKIAKDVYEVLK 4UA6 Chain:A ((5-262)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VQQKLAALEKS-S-GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKPAD-------LVNYNPIAE-KHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIG-DETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNTTGAASIRAGLPTSWTVGDKTGSGDYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESRRDVLASAARIIAEG--

General information: TITO was launched using: RESULT: Template: 4UA6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1178 7248 6.15 31.93 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 6.15 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_4UA6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UA6-query.scw PDB file : Tito_Scwrl_4UA6.pdb: