Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQMEMQLMAFELVDLDRQGTRMKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSKNPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVY-KTGIE--NFETARRLFLENKGELLPNSPIQYETATEMKSRFLECMSKYRE--HQTVVVVAHRMLMRQFVPN--------------EKIDFCQVIECELEI 3HJG Chain:A ((4-188)) -------------------KTLNIYLMRHGKVDAAPG----LHGQ--TDLKVKEAEQQQIAMAWKT-KGYDVAGIISSPLSRCHDLAQILAEQQLLPMTTEDDLQEMDFGDFDGMPFDLLTEHWKKLDAFWQSPAHHSLPNAESLSTFSQRVSRAWSQIINDINDNLLIVTHGGVIRIILAHVLGVDWRNPQWYSTLAIGNASVTHITITI

General information: TITO was launched using: RESULT: Template: 3HJG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 741 -48028 -64.81 -289.32 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -64.81 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_3HJG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HJG-query.scw PDB file : Tito_Scwrl_3HJG.pdb: