Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEIDRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLI-GYGVEQIISTGTCGVLAD-IEENAFLVPVRALRDEGASYHYVA-PCRYMEMQPEAIAAIEEVLEDRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELLFTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL 4M7W Chain:A ((26-258)) ---------------------PHIGANRGDVAETILLPGDPLRAKYIAETFLEDVV-Q-YNNVRGMLGFTGTYKGKKVSVQGTGMGVPSIGIYSHELITEFGVKNLIRVGTAGSYQEDVKVRDVVIAMSASTDSAINKLRFNGADYAPTASSDLVFKAYEIAKAKGLNVKAGNVFTSDTFYGDDPNAWKKWAEFGVLCVEMETAQLYTTAAKLGVNALTLLTISDSFITH-EVTSAEERQTTFNEMIEVALETALQL

General information: TITO was launched using: RESULT: Template: 4M7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1331 -50733 -38.12 -220.58 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -38.12 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_4M7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M7W-query.scw PDB file : Tito_Scwrl_4M7W.pdb: