Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVFVIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNVFITTTYAYAISRTTFKYRRFFTIFVLLSMLF--NAGLVPGYIMVTRVLQLG-----DTVWALIVPMLLS-PFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTALGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMAMVVVATLPIAILYPFFQRYFVKGLTIGGVKE 3FH6 Chain:G ((3-287)) --MVQPKSQKARLFITHLLLLLFIAAI-----------MFPLLMVVAISLRQRITPPPF----------------------PVLLWLWNSVKVAGISAIGIVALSTTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHGGVIFAYLGGIALHVWTIKGYFET-IDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVVFILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNY----------------LWGDFAAAAVMSALPITIVFLLAQRWLVN---------

General information: TITO was launched using: RESULT: Template: 3FH6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 806 -91733 -113.81 -372.90 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain G : 0.68 3D Compatibility (PKB) : -113.81 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.208

(partial model without unconserved sides chains): PDB file : Tito_3FH6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FH6-query.scw PDB file : Tito_Scwrl_3FH6.pdb: