Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIFIEYKIITLFISLKIKKGVFIMKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIKMYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYL-GWGDYGKKMSVITSSGENYDIAFADN---Y-IVNAQKGAYADLTELYKKEGKDLYKALD---PAYIKGN-TVNGKIYAVPVAANVASSQNFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPD------VVPFAIGKVFIPSDNFDYPVA---NGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSF---DLQQDTWFVREETVGPADY--GNSLLSRVANKDIQIKPITNFI-K-K-----NQTTQVANFVISNNSKNKEKSMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGN---THMGGW---N-T-GNNWILYI-NENVTDQQIEN-SKKELA-EAKESPALGFIFNT---DNVKSEISAIANTMQQFDTAINTGTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK 3A09 Chain:A ((7-489)) -----------------------------------------------------------TEKPLTLKIHMHFRDKWVW-DENWPVAREVARLTNVKLVGVANRAATNSQEQFNLMMASGQLPDIVGGDNLKDKFIRYGMEGAFIPLNKLIDQNAPNL-KAFFKTHPEVQRAITAPDGNIYYLPYVPDGLVSRGYFIRQDWLDKLHLKT--PQTVDELYTVLKAFKEKDPNGNGKADEIPFINRDPEE-VFRLVNFWGARSTGSNTWMDFYVENGKIKHPFAEVAFKDGIKHVAQWYKEGLIDPEIFTRKARSREQTFGNNIGGMTHD-WFASTALFNDALSKNIPGFKLVPMAPPINSKGQRWEEDARQIPRPDGWAITATNKNPVETIKLFDFYFGP-KGRELSNFGVPGLTYDIKNG-KPVYK-D-TVLKAAQPVNNQMYDIGAQIPIGFWQDYEYERQWTNDVALQGIDMYIKNKYVLPQFTGVNLTVEEREIYDKYWPDVKTYMFEMGQSWVMGTKDPEKTWNDYQQQLKNRG-FYQVMIVMQKAYDRQ-----

General information: TITO was launched using: RESULT: Template: 3A09.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2364 77714 32.87 177.03 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 32.87 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_3A09.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A09-query.scw PDB file : Tito_Scwrl_3A09.pdb: