Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFVNFFHEKEKIMRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPKYENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQDYLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLKILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTLLEDAKNKKSYDRLAICYVRIGICT---DDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQAKER 4YV9 Chain:D ((4-273)) ---------------ELGKTLRRLRQGKQVSISSLADEHLSKSQISRFERGESEISCSRLLNLLDKLNITIDEFVSTHS----THFFTLLSRVRKYYAEKNVAKLLKLLEDYAHKD--YESTMIKA-------ILSSIEP----TVEPSEEEVTRLTDYLFSVEQWGYYEIILLGNCSRFINYNTLFLLTKEMVTSFAYSEQNKTNKTLVTQLSINCLIISIDYSYFDHSHYLIEKIEFLLRDELNFYEKTVFLYVHGYYKLKQSGKDDMRQALQIFKYLGEDALYYSYKEHYRKE------

General information: TITO was launched using: RESULT: Template: 4YV9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 991 -145434 -146.75 -557.22 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain D : 0.66 3D Compatibility (PKB) : -146.75 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_4YV9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YV9-query.scw PDB file : Tito_Scwrl_4YV9.pdb: