Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKIYQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTP-------ISLIGAATDATDYVV-LAKALDKAAKEIGV---DFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTAVADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGVVKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPAVGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDEGMIAAVQNGSLNLEKLEAMTAICSVGLDMIAI-----PEDTPAETIAAMIADEAAIGVINMKTTAVRII-----PKGREGDMIEFGGL-LGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN----------- 5L3T Chain:B ((1-455)) MDMANQLLDELAHGNFSHLTLNLSQNGREIAILQKQLTGFDDKQLETFVEQHPAMPNDTRFKIMCTSFLNYARDVDPWSAWSSSDLIFEFYQCLINCLINDNAPHIEMLIPVATRETEFIINLAGKLDSFHLQLHTRSHQFLSHISSILSRLFNSIKP------PRGNASSTNI-------PGKQRILLYLVNKLNNIYFRIE--SPQLCSNIFKNFQPKS----MLAHFNEYQLDQQIEYRYLLGRYYLLNSQVHNAFVQFNEAFQSLLNLPLTNQAITRNGTRILNYMIPTGLIL-----------GKMVKWG----PLRPFLSQETIDNWSVLYKHVRYGN---------IQGVSLWLRQNERHLCARQLLIVLLEKLPMVTYRNLIKTVIKSWTTEWGQNKLPYSLIERVLQLSIGPTFEDPGAQEITIYNGIHSPKNVENVLVTLINLGLLRANCFPQLQLCVVKKTTMIQEIVPPVNERITKMFPAHSHVLW

General information: TITO was launched using: RESULT: Template: 5L3T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1672 9918 5.93 24.67 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 5.93 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.180

(partial model without unconserved sides chains): PDB file : Tito_5L3T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L3T-query.scw PDB file : Tito_Scwrl_5L3T.pdb: