Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFLNVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQ-RLVRKGEFDKYLTRP------INPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTSLKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRW-LISFIVPFAFTAYYPASYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS 5IMU Chain:A ((4-220)) AGAVSARAAEQQRLQRIVDAVARQ------EPRISWAAGLRD----DGTTTLLVT------DLAGGWIPPHVRLPANVTLLEPTARRRDADVIDLLGAVVAVAAHESNTYVAEPGPDAPALTGDRSARSAIPKVDEFGPTLVEAVRRRDSL--------PRIAQAIALPAVRKTGVLENEAELLHGCITAVKESVLKA---------YPSHELTAVGDWMLLAAIEALIDEQDYLANYHLAWYAVTTRRG-----------------------------

General information: TITO was launched using: RESULT: Template: 5IMU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 792 -46485 -58.69 -233.59 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -58.69 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.274

(partial model without unconserved sides chains): PDB file : Tito_5IMU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IMU-query.scw PDB file : Tito_Scwrl_5IMU.pdb: