Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGVLPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAETPVLSSLDEKLVDEAIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDGQ--LARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAEKVEAGQLDFLHPLEI-GTGDLVKVFLTPEEATEVRFGRFIELDQTDKEL-AAFEDDKLLAILEKRGNLYKPRKVFS 1SGV Chain:B ((5-283)) GPGIVVIDKPAGMTSHDVVGRCRRIFATRRVGHAGTLDPMATGVLVIGIERATKILGLLTAAPKSYAATIRLGQTTSTEDAEGQVLQSVP-AKHLTIEAIDAAMERLR----------------------------SVQLEARPIRIDRFELLAA---RRRDQLIDIDVEIDCSSGTYIRALARDLGDALGVGGHVTALRRTRVGRFELDQARSLDDLAERP-----ALSLSLDEACLLMFARRDLTAAEASAAANGRSLPAVGIDGVYAACDADGRVIALLRDEGSR--------

General information: TITO was launched using: RESULT: Template: 1SGV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1296 -44207 -34.11 -178.97 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -34.11 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_1SGV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SGV-query.scw PDB file : Tito_Scwrl_1SGV.pdb: