Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEIILGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEAYDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHRIKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE 1VEZ Chain:B ((5-169)) ------------------ILRMGDPILRKISE----PVTEDEI----QTKEFKKLIRD--MFDTMRHAEGVGLAAPQIGILKQIVVVGSEDNERYPGTPDVP---ERIILNP-VITPLTKDTS-GFWEGCLSVP-GMRGYVERPNQIRMQWMDEKGNQFDETIDGYKAIVYQHECDHLQGILYVDRLKDTKLFGFNETL----

General information: TITO was launched using: RESULT: Template: 1VEZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 838 -66428 -79.27 -402.59 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -79.27 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_1VEZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VEZ-query.scw PDB file : Tito_Scwrl_1VEZ.pdb: