Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDALYELVP-QEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQG------VEGLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNL-PDYTYPVFGMALGVPN-QTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQFGQAEPSSTRKNLEQKKLL-- 1BKJ Chain:A ((2-240)) -NNTIETILAHRSIRKFTAVPITDEQRQTIIQAGLAASSSSMLQVVSIVRVTDSEKRNELAQFAGNQAYVESAAEFLVFCIDYQRHATINP----------DVQADFTELTLIGAVDSGIMAQNCLLAAESMGLGGVYIGGLRNSAAQVDELLG-LPENSAVLFGMCLGHPDQ-NPEVKPRLPAHVVVHENQYQELNLDDIQSYDQTMQAY-----YSTWSQEVTGKLAGESRPHILPYLNSKGLAKR

General information: TITO was launched using: RESULT: Template: 1BKJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 844 -35628 -42.21 -162.68 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -42.21 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_1BKJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BKJ-query.scw PDB file : Tito_Scwrl_1BKJ.pdb: