Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYDAPSCPECGSQMKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQIPRIINQKIAQKLIEK-ISMTDIAHQLSISTSTVIRKLNDFHFKHDFSCLPEIMSWDEYAFTKGKMSFIAQDFNNLNIITVLEGRTQAVIRDHFLKYDRAVRCRVKIITM--DMFSPYYGLAKQLRFHIVQHLSRAMSRVRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSDKQFYRPTFRMHLTNKEILNKLLSYSEDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLK-----------------------DKEKIVNALQLHYSNAKPEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKE-------RTKFVLSRA- 2VFR Chain:A ((4-421)) --FMSDITVTNWAGNITYTAKELLRPHSLDALRALVADSARVRVLGSGH-------SFNEIAEPGDGGVLLSLAGLPSVVDVDTAARTVRVG----------GGVRYAELARVVHARGLALPNMASLPHISVAGSVATGTHGSGVGNGSLASVVREV-ELVTADGSTVVIARGDERFGGAVTSLG-ALGVVTSLTLDLEPAYEMEQHVFTELPLAGLDPATFETVMAAAYSVSLFTDWRAPGFRQVWLKRRTDRPLDGFPYA--APAAEKMHPVPGMPAVNCTEQFGVPGPWHERLPHFRAEFTPSSGAELQSEYLMPREHALAALHAMDAIRETLAPVLQTCEIRTVAADAQWLSPAYGRDTVAAHFTWVEDTAAVLPVVRRLEEALVPF--AARPHWGKVFTVPAGELRALYPRLADFGALAGALDPAGKFTNAFVRGVLA

General information: TITO was launched using: RESULT: Template: 2VFR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2249 -41558 -18.48 -108.22 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -18.48 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.153

(partial model without unconserved sides chains): PDB file : Tito_2VFR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VFR-query.scw PDB file : Tito_Scwrl_2VFR.pdb: