Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVDRVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLLDMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVEENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGDKVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS 1KD8 Chain:A ((1-35)) -------------------------------------------------------EVKQLEAEVEEIESEVWHLENEVARLEKENAECEA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1KD8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 5 -840 -168.00 -24.00 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : -168.00 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.596

(partial model without unconserved sides chains): PDB file : Tito_1KD8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KD8-query.scw PDB file : Tito_Scwrl_1KD8.pdb: