Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRNNSQNLIMLKHDFASHIEDLKCATKPRSEALGRHLWTCTIDADFYWLKFHLPNVHAQSEQDFLHELQFYEDITYKKANWLLPFKIIEGRTISQQTQFQGKVLVLPDTEC--WFDDLDQKQNLKNINEKIYLALVK-----LAELHELGWIHGDIKKEHFRKFKQE-----LYLIDFEKTRLISSPDPITD--ATPRYMAPELFHGA------NKTVQSDLYALGIVLYEWLT-----------------QTRLQANSYHEWAVLHCQKLNVELPSSFQI-------------FLPLLSGLLQKQQQNR--FSNVHEAINCLKALST--------------------------------------------------------------------------- 4EUT Chain:A ((2-387)) --GSQSTSNHLWLLSDILGQGATANVFRGRHKK-----------TGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKN--------IVKLFAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGMNHLRENGIVHRNIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMYERAVLDHQKKYGATVDLWSIGVTFYHAATGSLPFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVLANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSRE

General information: TITO was launched using: RESULT: Template: 4EUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1093 14391 13.17 55.99 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 13.17 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_4EUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EUT-query.scw PDB file : Tito_Scwrl_4EUT.pdb: