Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKLLKIMQELR-EKCPWDQEQTSMSLTKYAIEEAYEVEAAIRQGD---------INEIRNELGDLLLQVVFQSQMFSEQGAFNFQDVVEAISEKLVRRHPHVFQADQFNNLIPEQVSELWKQIKQQEKQGKPQSRLDEIKHGPALSQAQEIQKNVAKVGFDFETVEDAYTKLEEELDEFKQALKNQNSDEIQDEFGDCLFSLVNVGRKLGISSESSLLSTIHKFRSRFAFIEEQAIKQQRTLEDMTLSEMDELWNQAKRQLKSGEKPHAIQHEILEK 2Q73 Chain:C ((6-91)) ------LQSHIKEFDYAPE---QSEHYFFKLIEEVGELSESIRKGKSGQPTLDELKGSVAEELYDVLYYVCALANI----HGVNLEKTHELKEVLNKVK-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Q73.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 205 -15182 -74.06 -199.76 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain C : 0.62 3D Compatibility (PKB) : -74.06 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_2Q73.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q73-query.scw PDB file : Tito_Scwrl_2Q73.pdb: