Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAANVIVYSTSVCPYCVRAKQLLERKGVAYKEV---NLSVEAPEVRAELMQRTNHRTVPQIFINDQFIGGFDQLYALEREGKLDELLA 3RHC Chain:B ((20-104)) ---TVVIYSKTWCSYCTEVKTLFKRLGVQPLVVELDQLGPQGPQLQKVLERLTGQHTVPNVFVCGKHIGGCTDTVKLNRKGDLELMLA

General information: TITO was launched using: RESULT: Template: 3RHC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 315 -38534 -122.33 -469.93 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -122.33 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.739

(partial model without unconserved sides chains): PDB file : Tito_3RHC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RHC-query.scw PDB file : Tito_Scwrl_3RHC.pdb: