Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLKNAKIKILDLDLRGCLYLNNFSHSQRVALFFKIISRAGDGPFWYLMLAIVWGMQGITYSLQIIYLLLGGSVGTAIYKFLKHK--TTRPRPYQVHQVIV-L---GERPLDHFSFPSGHTLHAVMVTIVLGYIQPVL-----------------------------LAAMLPFMVLVALSRMVLGLHYPSDVIVGALIGAAVASLIIFVAPLFGIAL 1QI9 Chain:B ((377-509)) ------------------------------------------------------------------------------AELLKRVAAINAAQNPNNEVTYLLPQAIQEGSPTHPSYPSGHATQNGAFATVLKALIGLDRGGDCYPDPVYPDDDGLKLIDFRGSCLTFEGEINKLAVNVAFGRQMLGIHYRFDGIQGLLLGETITVRTLHQE-------

General information: TITO was launched using: RESULT: Template: 1QI9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 157 -18129 -115.47 -184.98 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.54 3D Compatibility (PKB) : -115.47 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.277

(partial model without unconserved sides chains): PDB file : Tito_1QI9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QI9-query.scw PDB file : Tito_Scwrl_1QI9.pdb: