Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MAYDMVSSTNLDKNIIGVHLMIHSKIIG-IGVITGLCSMAALAEPAVQPGDTLESLSKVKVTTTVSGQPGSISDLVNSGQVQQVSAAPTDAVPPAPNQAAPAPQDPNTPPPAANPNQSADPMAKDGALPADAPMPQAQ 5LPI Chain:A ((6-134)) EHYLDNLCIFCGERSEEFTEEGLDLHYWKHCLMLTRCDHCKQVVEISSLTEHLLTECDKKDGFGKCY----------------RCSEAVFKEELPRHIKHKDCNPAKPEKLANRCPLCHENFSPGEEAWKAHLMGPAGCTMNLRK

General information: TITO was launched using: RESULT: Template: 5LPI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 420 35302 84.05 291.75 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 84.05 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_5LPI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LPI-query.scw PDB file : Tito_Scwrl_5LPI.pdb: