Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQNLPIIAVLYMILSMVSYQISASFAKQLIAALDPLTVTILRLCFAAVIVAVMFRSWKIWSRLPYLKWRDLLMYCASLGLMNILFYTSLGKLPQGIAVGLEFIGPLGLALVSIKQKSEYIWVAFAMLGIALMVPWQDPTQHHFSYVGAACALGAGLCWAFYIYFGQKVVTQNIGMHALTIGITVSA-LALLPIGIWYNGPALLDTQYWGKALLIAVLATAIPYALDLMALKQLNKLTYGTLTSLAPALAALTGFLLLHEEISMLQWVALACVMLASIGVTLRSKTAS 2YHI Chain:C ((190-221)) ------------------------------------------------------------------------------------------------------------------------------------------------------------CMAFSLY--------NMGKHAL-VGLTQSAALELAPYGIRVNG----------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2YHI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 24 -2561 -106.71 -82.61 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain C : 0.41 3D Compatibility (PKB) : -106.71 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.41 QMean score : -0.032

(partial model without unconserved sides chains): PDB file : Tito_2YHI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YHI-query.scw PDB file : Tito_Scwrl_2YHI.pdb: