Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHTALDQLKTLTTVVADSSDLEAIRKFRPLDATTNPSLITAAAEQPESKELIEDAYYQAKEEG--YKNDELI-ERTIDILTVKFGVEILKLIEGRVSTEVDTALSYDTEATIQKAHELCELYKGYGIDQSRILIKIASTWEGIQAAKVLEAEGIACNLTLLFGLHQAQACADAKVTLISPFVGRILDWYKKAEGVDSYP-IEKDPGVVSVKKIYTYYKQQNIPTQVMGASFRSIDQVLGLAGCDLLTISPSLLTQLEQDTRTVDAALDANKAKQAEAIVR-PAQDEQSFKDELNHDLMAFQLLQGGVDGFIKARDQLSLLLRQSFGIDAEIKS 3TKF Chain:B ((25-345)) MQKSVLEQLKQVTMVVADTGDFELIKKYKPVDATTNPSLILKAVKEQKYSNLVAETISKVKANNPDLNSDDLVKEIAIEIL-VSFGIKILDVIEGKVSSEVDARVSFNSATTIDYAKRIIARYESNGIPKDRVLIMIAATWEGIKAAKLLQKEGINCNLTLIFDKAQAKACAEAGVYLVSPFVGRITDWQMQQNNLKTFPAIADDDGVNSVKAIYKLYKSHGFKTIVMGASFRNVEQVIALAGCDALTISPVLLEEL----KNRDEHLEVKLT--------SPQISEADFRWLMNENAMATHKLAEGIRLFTKDTIELENIIKQNL--------

General information: TITO was launched using: RESULT: Template: 3TKF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1725 -19322 -11.20 -62.73 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -11.20 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_3TKF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TKF-query.scw PDB file : Tito_Scwrl_3TKF.pdb: