Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKFNNNQSDNLFSQVFGVAKKLSSTGLNILQQSQIGEVSKLVEPLSNGKTVEGSARNKSPFEVEQYESPLQMLREHLPKVTRQVFGRHFRKVNGIATFISPDWNEKISSYLFDWLNDFSSK---------STLTEKILEEAGAKDLFELTKDTSRSQRLSQALIEQNKLIASIQGAITGVSG-MVGAAVDIPVSLVLVLRTIYQTGRSHGFDLTEATDQDVVEFIFKEVDI-SLIA-------EKQTLLLALKALRNMLETQDIQQFQQVLGSSNDIETL--KSWLVDENGKFKWNWLNKVPQLAVVGKFTPVAG--AVLSAVYSWKLQEDVGHKAQAIFGAARHYLNEHPNEHLSPLQAYYAAVTLIQKASPR-LLNVGENGSVHATQHHKIENHDVISKVSVVVKSNTSEKSEEKVQ--------ENVHQGIEHLAEKHVVEHEHSEQKPALE------------PESEENDDVIEGQKYS-- 5L3T Chain:B ((1-455)) -----MDMANQLLDELA----HGNFSHLTLNLSQNGREIAILQKQLT------GFDDKQLETFVEQHPA---MPNDTRFKIMCTSFLNYARDVDPWSAWSSSDLIFEFYQCLINCLINDNAPHIEMLIPVATRETEFIINLAGKLDSFHLQLHTRSHQFLSHISSILSRLFNSIKPPRGNASSTNIPGKQRILLYLVNKLNNIYFRIES----------PQLCSNIFKNFQPKSMLAHFNEYQLDQQIEYRYLLGRYYLLNSQVHNAFVQFNEAFQSLLNLPLTNQAITRNGTRILNYM--IPTGLILGKMVKWGPLRPFLSQETIDNWSVLYKHVRYGNIQGVSLWLRQN-ERHLCARQLLIVLLEKLPMVTYRNLIKTVIKSWTTEWGQNKLPYSLIERVLQLSIGPTFEDPGAQEITIYNGIHSPKNVENVLVTLINLGLLRANCFPQLQLCVVKKTTMIQEIVPPVNERITKMFPAHSHVLW

General information: TITO was launched using: RESULT: Template: 5L3T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1715 -5561 -3.24 -13.56 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -3.24 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_5L3T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L3T-query.scw PDB file : Tito_Scwrl_5L3T.pdb: