Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLNHFATELDELKQQGNFRQFT--QNVQHGRFI-------TIQNKTMLNLASNDYLGLAADINLRQEFLDSYPLERSYFSSSSSRLLTGNFDEYEQLENSLSQAFSGRAALLFNSGYHMNIGILPAVA--DSKTLILADKLVHASMIDGIRLSGAQYVRYRHNDLQHLEQLLQKYHDDDQIERIIVVTESIFSMDGDETDLTALVQLKKRFAKTMLYVDEAHSIGVRGQQGLGCAEQYNVIQEIDFLVGTLGKALAAVGGYIICDQIIKDYLINKMRPLIFSTAQPPIVMAWANFIFKKVLMA--QSQREHLKNISQYLQQAVVQKGYVS-PSTSHIIPVIVGESQATIDKAKQVQ-ESGFYAMPVRPPTVPKNSSRLRISLTSMVEQHELEALVSFL 2BWN Chain:E ((2-393)) -DYNLALDKAIQKLHDEGRYRTFIDIEREK-GAFPKAQWNRPDGGKQDITVWCGNDYLGMGQHPVVLAAMHE--ALEAVGAGSGGTRNISGTTAYHRRLEAEIAGLHQKEAALVFSSAYNANDATLSTLRVLFPGLIIYSDSLNHASMIEGIKRNAGPKRIFRHNDVAHLRELIAADD---PAAPKLIAFESVYSMDGDFGPIKEICDIAEEFG-ALTYIDEVHAVGMYGPRGAGVAERDGLMHRIDIFNGTLAKAYGVFGGYIAASARMVDAVRSYAPGFIFSTSLPPAIAAGAQASIAFLKTAEGQKLRDAQQMHAKVLKMRLKALGMPIIDHGSHIVPVVIGDPVHTKAVSDMLLSDYGVYVQPINFPTVPRGTERLRFTPSPVHDLKQIDGLVHAM

General information: TITO was launched using: RESULT: Template: 2BWN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 2117 935 0.44 2.48 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain E : 0.75 3D Compatibility (PKB) : 0.44 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_2BWN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BWN-query.scw PDB file : Tito_Scwrl_2BWN.pdb: