Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTLKNDRFLRALLREPVDTTPIWMMRQAGRYLPEYRETRSKAG---DFLSLCKNTEFACEVTLQPLRRYDLDAAILFSDILTIPDALGLGLYFETGEGPKFHKTVRTEQDVANLPKLNAKADLDYVMNAVSTIRSALGGQVPLIGFSGSPWTLATYMVEG--GS---SKEFRFTKQ-MMYAQPEVLHALLDHLADSVIDYLNAQIDAGAQAIQIFDSWGGALAHREYVEFSLNYMNKIIAGLQREKDG----RRIPVIVFTKGGGQWLEPMITTGADALGLDWTTPLNTARNV-VSGRVALQGNLDPAVLYGS-AASIEKAVKAMLDDAYANGEKTGYVANLGHGITQWVDPAQPKIFVDTVHEYSAKYLG 4ZR8 Chain:B ((12-365)) ---LKNDRFLRALLREPVDTTPIWMMRQAGRYLPEYRE--TR-SKAGDFLSLCKNTEFACEVTLQPLRRYDLDAAILFSDILTIPDALGLGLYFETGEGPKFHKTVRTEQDVANLPKLNAKADLDYVMNAVSTIRSALGGQVPLIGFSGSPWTLATYMVEGGSSKEFRF------TKQMMYAQPEVLHALLDHLADSVIDYLNAQIDAGAQAIQIFDSWGGALAHREYVEFSLNYMKKIIAGLQR---EKDGRRIPVIVFTKGG-GQWLEPMITTGADALGLDWTTPLNTARTTVAG-RVALQGNLDPAVL-YGSAASIEKAVKAMLDDAYANGEKTGYVANLGHGITQWVDPAQPKIFVDTVHEYSAKYLG

General information: TITO was launched using: RESULT: Template: 4ZR8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1937 -38291 -19.77 -112.95 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.95 3D Compatibility (PKB) : -19.77 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_4ZR8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZR8-query.scw PDB file : Tito_Scwrl_4ZR8.pdb: