Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIQQALNHITKNIHLTQPQMEEIMRSIMQGEATEAQIGALMMGLRMKGESIDEMTAAARVMREFAIKIDVSDIKHLVDIVGTGGDGQNLFNVSTASSFVIAAAGATIAKHGNRGVSSKSGSSDLLEQAGIHLDLDMQQTERCIREMGVGFLFAPNHHKAMKYAAGPRRELGIRSIFNLLGPLTNPAGVKRFVIGVFSDELCRPIAEVMKQLGAEHVMVVHSKDGLDEISLAAPTTIAELKDGEITEWTLNPEDVGIESQTLNGLVVADATASLKLIKDALSKNKSDIGEKAANMIALNAGAGIYVAGITKTYAQAVVFAQDIIYGGQTLEKMSVLAEFTKTLKQSQAD 2ELC Chain:D ((21-255)) -----------------------VMRALMAGEVSPVRAAGLLVALSLRGERPHEIAAMARAMREAARPLRVHR-RPLLDIVGTGGDGKGLMNLSTLAALVAAAGGVAVAKHGNRAASSRAGSADLLEALGVDLEAPPERVGEAIEELGFGFLFARVFHPAMRHVAPVRAELGVRTVFNLLGPLTNPAGADAYVLGVFSPEWLAPMAEALERLGARG-LVVHG-EGADELVLGE-NRVVEVGKG---AYALTPEEVGLKRAPLEAL------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ELC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1224 -126103 -103.03 -536.61 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain D : 0.76 3D Compatibility (PKB) : -103.03 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.483

(partial model without unconserved sides chains): PDB file : Tito_2ELC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ELC-query.scw PDB file : Tito_Scwrl_2ELC.pdb: