Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVIALFSKNWSRTMSATIVNTTDDNFQADVLDAETPVLVDFWAGWCAPCKAIAPVLEDLSSEYAGKVKIVKVDVTSCEETAVKYNIRNIPALLLFKNGEVVAQQIGAVPRSKLVSFIDENV 2FCH Chain:C ((3-107)) ----------------KIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRSIPTLLLFKNGEVAATKVGALSKGQLKEFLDANL

General information: TITO was launched using: RESULT: Template: 2FCH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 458 -83991 -183.39 -799.91 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain C : 0.82 3D Compatibility (PKB) : -183.39 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.834

(partial model without unconserved sides chains): PDB file : Tito_2FCH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FCH-query.scw PDB file : Tito_Scwrl_2FCH.pdb: