Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPGPRDALKHMLAYGERDGFKVKNV--DNYAGHI-DLGEGDETLGIFGHMDVVPAG--DGWDTDPYEPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGW-GDMAYYFEH----EEEPDFGFSPDAEFP------IINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVPGTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQSGINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAGLFAFEE---NGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGA--RVMEPHYVPMDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLK----RGVAYGAMFPGYTDTMHQANEFMSLDDLFRATAIYADAIYRLAK 5UEJ Chain:B ((5-380)) -----------TETQSLELAKELISRPSVTPD------------DRDCQKLLAERLHKIGFAAEELHFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADMKTSIACFVTACERFVAKHPNHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYCIVGEPTAVDKLGDMIKN---------------------------------------------------------------------GRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQE-----VWD---E---------------GNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAILDKHGVQYD-LQWSCSGQPFLTQ-AGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKAIAQELIELGPSNA----TIHQINENVRLNDIPKLSAVYEGILARLLA

General information: TITO was launched using: RESULT: Template: 5UEJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1884 62336 33.09 177.59 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 33.09 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_5UEJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UEJ-query.scw PDB file : Tito_Scwrl_5UEJ.pdb: