Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQTQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDE-KDAIEIQRLYLLKDYQNKGLGQRLLDEANRYAKTNKNVIYV 3FNC Chain:A ((4-115)) --MDFHIRKATNSDAEAIQHVATTSWHHTYQDLIPSDVQDDFLKRFYNVETLHNRI--SATPFAVLEQADKVIGFANFIELEKGKSELAAFYLLPEVTQRGLGTELLEVGMTLFHV-------

General information: TITO was launched using: RESULT: Template: 3FNC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 432 -52647 -121.87 -474.29 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -121.87 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_3FNC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FNC-query.scw PDB file : Tito_Scwrl_3FNC.pdb: