Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSEALVVIEADVFA-----HFDYGFRVF-----GLSVAEFKQYEAQFLPILDQVIKNKLAFEL--NAKSAYLYDNLALY---------EYVIDLCLSRGGTLF-- 5MLC Chain:L ((55-250)) TKCTEEWRQLKEAVKKEFAIPHVPLDQRWMFTLEEATGPDIWN--TTWYPKSADHVPTDK---KWYVVDATDLILGRMASTIAIHIRGKNLASYTPSV--DMGAFVIVVNADKVAVSGKKRTQKLYRRHSGRPGGLKEETFDQLQKR---IPERIIEHAVRGMLPKGRLGRYLFNHLKVYKGAEHPHQAQQPIDLPLRDKRIRVEK

General information: TITO was launched using: RESULT: Template: 5MLC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 518 7252 14.00 42.41 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain L : 0.68 3D Compatibility (PKB) : 14.00 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.141

(partial model without unconserved sides chains): PDB file : Tito_5MLC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MLC-query.scw PDB file : Tito_Scwrl_5MLC.pdb: