Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDNKHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEEFSQEEHLVFICGHYEGYDER-IRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVLGNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQRRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK 4YVK Chain:B ((21-263)) MWIGVISLFPEMFKAITEFGVTGRAVKHNLLKVECWNPRDFTFDKHKTVDDRPYGGGPGMLMMVQPLRDAIHTAKAAA-GEGAKVIYLSPQGRKLDQGGVTELAQNQKLILVCGRYEGIDERLIQTEIDEEWSIGDYVLTGGELPAMTLIDAVARFIPGVLGKQASAEEDSFADGLLDCPHYTRPEVLEGLTVPPVLMSGHHEEIRKWRLKQSLQRTWLRRPELLEGLALTDEQRKLLKEAQAE----------

General information: TITO was launched using: RESULT: Template: 4YVK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1022 -91752 -89.78 -379.14 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -89.78 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_4YVK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YVK-query.scw PDB file : Tito_Scwrl_4YVK.pdb: