Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHENSDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHE-----DNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFG---FPFL-ADYELRGTPSDVTRIYQILAENNK 3HR4 Chain:A ((43-181)) VTILFATETGKSEALAWDLGALFS-CAF---NPKVVCMDKYR--LSCLEEERLLLVVTSTFGNG----------DCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYP-RFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTF-

General information: TITO was launched using: RESULT: Template: 3HR4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 653 -37445 -57.34 -288.04 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -57.34 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_3HR4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HR4-query.scw PDB file : Tito_Scwrl_3HR4.pdb: