Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -FGRRRDGRMPPWEW--VTWKDFCRLTLKKGDEIQSQSKWVAKPGPIGILIQPGVYESFKTDKDCNPKIRRGKLPDGYTLHGQLKYIT-PDLASMYNRVLDDTKWWPFDSQEAPQTSTTVEKSL 1FU6 Chain:A ((1-111)) GMNNNMSLQDAEWYWGDISREEVNEKLRDTAD-----GTFLVRDASTK---MHGDYTLTLRKGGNNKSIKIFHRDGKYGFSDPLTFNSVVELINHYR----NESLAQYNPKLDVKLLYPVSKY-

General information: TITO was launched using: RESULT: Template: 1FU6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 452 37376 82.69 349.31 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 82.69 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.191

(partial model without unconserved sides chains): PDB file : Tito_1FU6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FU6-query.scw PDB file : Tito_Scwrl_1FU6.pdb: