Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIFNKMKKDKIFILLGLFGTSVYAAEPITSSTDAVIRQQQRDEALQKQIQPTATVQTGLEKQLQSKPQLQFLKSHSEDICFEIKKFILIGDDARRFTFAMRP---------ITQGEH-NLVGRCIGVQGLNQALDLIQNSI-ISHGYVTTRMLLPQQNIA---SGEIKLQVIAGKV-DQIKFVEGTSKRAHKFNALPVKSGDILNVRDIEQGLENFKRVPTVEADFKIKPSEQKTAPGYSDLELAWQQSKPYRIHLGIDDAGSD----STGKYQGTATLSLDHLFTANDLFYGSYNH-DLGGGDSGKRGT-DGYYLSYSIPYHYWLL-STSYSQSNYNQTVAGASESYNYSGESKLIGADLSRVLYRDAKRKTTASVGGWYRESQNYINDVEVEVQRRKTAGWKASLDHSEYLSHATLNGNVTYKRGT-GAFNAMYAPEEDFGEAYTRVGIWQANASLQVPFKVGQQNLQYLAEWRMQHSKKPLTPQDRFSIGNRYTVRGYDGEQTLLADNGFLVRNELSGSIPKLP-MQWYSGIDYGEVGGTTAHKPNPLVGTSLLGAVIGLRGQVFKSVSYDAFVGAPLKKPEYFKTDDFTTGFSVNWMY 4R7F Chain:A ((2-398)) ------------------------------------QVKFFTDVNSKQIK---TLQVKVAGELISEPYIALGGEEQIEINFDGL-----GSGYTRYAYNVVHCNADWTQSQLSPIEYMN------GFQGTT--IDDFANSIGTTTQYSNYRLLLPNDDVQFKVSGNYAIQVYNEDTPDQIIFTACFSVVEPVVNISASVSGNT----DIDTN------QSHQQVSFNINNKNFPITYPQTDLKIFVYQDN------RRDNAVTDLQPMSILENQISYTYNRNLIFPAGN----EYRRMEF---LSNKYNGMHVENISFHNPYYNVELMTDYRRDKG----------TYQYDQDQD------GRFFIRCSDCND--------PDTEADYYIVHFTLACDPLPDGSVYLN-------GELFNNVLDEKSKMG-----YNFE-------------TKQYEKAVLLKQGSYNYQYLFVPT----------------GSSV------GQTGPIEGNYYQTQNEYSIYVYYRPMGA-----------------------------------------RYDRLIGVTTVRN------------------

General information: TITO was launched using: RESULT: Template: 4R7F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2006 93802 46.76 251.48 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 46.76 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.124

(partial model without unconserved sides chains): PDB file : Tito_4R7F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R7F-query.scw PDB file : Tito_Scwrl_4R7F.pdb: