Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMKLLMTSVLSTSLFLVACGGGSSDDGPATTNPSGTPTNNIQNPVVKVEAYTSTNLGSVAAESSILTYKMLGQSGQEVQATSLVFTPNTPPPVGGWPIVVWAHGTTGVADACAPSKAALADSTKDLISKLLAAGYVVVAPDYEGLGTPGIHPFL---------NV-----KSEAFSITDAVVAARNYLSQRNLLTSKKWGTVGHSQGGHAALGAAQYANRAQLDYKGTVAVAPASNWGSILVDGEAQVANAPIDIKIGTYAQLDTYTALVTAGIRNTQPSFDYGQVFTSQISSIAAQAENLCSGPLYGAFYEGMSNYAKDHNGTLDGFTRTQPNFMAVPLVKTFLEKDSQPLQVKVTTPIIIYQGIADPTVPKLA--TDLLISNATVVGTKINSYVTGNWDHGTAM-SSNVDNIVGNVQTLLSAQ 3FCX Chain:A ((25-281)) -------------------------------------------------------------------------ELNCKMK--FAVYLPPKAE-TGKCPALYWLSGLTCTEQNFI--------SKSGYHQSASEHGLVVIAPDTSPRGCNI-----FGTGAGFYVDATEDPWKTNYRMYSYVTEELPQLINANFPVDPQRMSIFGHSMGGHGALICAL---KNPGKYKSVSAFAPICNPVLCPWGKKA---------------------------F---------SGYLG--------------------------------------------------SKWKAYDATHLVKSYP---LDILIDQGKDDQFLLDGQLLPDNFIAACTEKKIPVVFRLQEDYDHSYYFIATFITDHIRHHAKYLN--

General information: TITO was launched using: RESULT: Template: 3FCX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1275 -32135 -25.20 -142.19 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -25.20 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.371

(partial model without unconserved sides chains): PDB file : Tito_3FCX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FCX-query.scw PDB file : Tito_Scwrl_3FCX.pdb: