Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MFT------------------KKDLLQQRMLIDQL-RTQNSLSPQNAAKLGQSIASSWERSASAAIPKERFAAPLLEKKSASQNALD-LALSQCADDLRHIAEQ------------SSMVIAVGDIGSTIIWTASS--------------------------AQMQSAAERVHFVQ------GGQWR-EEFVGTNALA----LSLKTQQSSCVFSNEHYMESI--HDWVCYAAPIIDPYSKQ-------TLGVVDLSTTWKNHNTLGILAAERCASIIQSALLEQQRQQLHIRAFSTPQVKFNGKS-LLLTPRQIEILTILALCPHGLTLENLY---QALYGERKVSMGTLKAEMSQLR-DILGG-LLGSRPYRLLVHVEADFLQAEQALDAGYVASALQLYAGVFLAKTESPFLCAWRDCL---ESRLSDAIFKAQETDL-----------LLKHLAHFPEAIDAVERLMELL-------------------PSEHPAHQLLVKYIDSPKLS---------------------------------------------------------------------------- 4Y9J Chain:A ((1-592)) TITARHTQYSHAKTGGFSQTGPTLHNPYKDDPILDRTLRRLLPESEYMRVAADLSKFGDRITSEVEHLGRQAELEQ----PRLEHQDAWGKRVDKLIVCNEWHKLKQICAEEGVISIGYEDSVDPFVRRIHQVAKLFLFSPSAGLVSCPMAMTDGAVKTLTSLNLYGKHKLATEAVDRLRSRDPSKAWTSGQWMTEKKGGSDVAGGCDTYAVQIDKDTYRLHGYKWFSSAVDADVALTLARIVDSDGNALEGSRGLSLFLLKIRDESGNLNGIQMVRLKNKLGTKQLPTAELLLDGAIAERIGDQGRGVAGISNMLNITRIHNAVASLGYMRRIISLARDYSTKRVVFGQTQSKWPLHTTTLAKMEVDTRGSMLLLFEAARLLGLSEAGKSSDVEAMMLRLITPVLKLYAGKQAVPMVSEGIECFGGQGYMEDTGLPTLLRDAQVTPIWEGTTNVLSLDVLRVFSGKENILLAFGKRVEQLLGNTKTEDEKLKKSKEAVESALKQLQKLLVKASDSAIQGETRIDSVARHIAFTIARIYSGALLIDHASDSSVANQSDIEVAYRYCCEQPLIDLRWEWFASERVKADREIVFDNFT

General information: TITO was launched using: RESULT: Template: 4Y9J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1899 9054 4.77 23.10 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 4.77 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.253

(partial model without unconserved sides chains): PDB file : Tito_4Y9J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y9J-query.scw PDB file : Tito_Scwrl_4Y9J.pdb: