Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------MTDLNKKREVNLSFEQDD--GAVWVFDGDSHQGTEISHLMMMHSDEYNED---------ELRVICNHAAFEIDRLRAELEKAKGQAV----PDSSHGVILTCEQLRDALEFSAPDLNIESNE--FSDEQMGTELAIIYQESGHSGEGFYSYYVECPDEGSIKLGES--ESGAEG--------------- 5FT3 Chain:B ((1-221)) MTKLILYTLHVSPPCRAVELCAKALGLELEQKTVNLLTKEHLTPEFMKMNPQHTVPVLDDNGTIVCESHAIMIYLVSKYGKDDSLYSKELVKQAKLNAALHFESGVLFARLRFVCEPILFAGGSEIPADRAEYVQKAYQLLEDTLVDDYIVGNSLTIADFSCVSSVSSIMGVIPMDKEKFPKIYGWLDRLKALPYYEAANGSGAEQVAQFVLSQKEKNAQK

General information: TITO was launched using: RESULT: Template: 5FT3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 550 14329 26.05 92.45 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : 26.05 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_5FT3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FT3-query.scw PDB file : Tito_Scwrl_5FT3.pdb: